融合菌株F14降解菲过程中细菌表面性质变化研究

融合菌株F14作为高效降解PAHs的融合菌株,它是由假单胞菌和鞘氨醇单胞菌作为亲本,通过原生质体融合技术融合而成。为进一步了解降解菌对污染物的降解机理,从微生物细胞本身考察了降解菲过程中细胞表面物质及疏水性的变化。结果表明:细胞膜表面脂多糖含量、磷脂及脂质过氧化物与降解菲的浓度具有密切的关系。随着菲的浓度增大,磷脂及脂多糖含量也随之增大;当浓度大于150 mg/L时,磷脂含量减小,脂多糖含量继续增大,丙二醛的含量随着菲浓度的增大而增大。融合菌株F14具有较高的表面疏水性,处于对数期以及稳定期的F14表面疏水率达到69.7%,65.2%。研究融合菌株降解菲过程中细菌细胞膜上表面性质的变化,有利于更深入的了解F14对菲的降解机制。

Study on Characteristics of the Cell Surface Features of Fusant Strain 14 in the Process of Degrading Phenanthrene

Fusant strain 14 is a high-efficiency PAHs-degrading bacterium by protoplast fusion between Sphingomonas sp. GY2B and Pseudomonas sp. GP3A, in order to examine the surface properties mechanism of F14 during its degrading process, from the perspective of individual cells of the bacterial explored to the changes of the surface material and cell hydrophobicity in the process of degradation. Results showed that the phospholipid, lipid hydroperoxide, lipopolysacharide and the hydrophobicity of cell membrane of the F14 were related definitely with the degradation of phenanthrene. With the increase of the concentration of phenanthrene, the content of phospholipids and lipopolysaccharide were increased, when the concentration is greater than 150 mg/L, the phospholipids is reduced. Malondialdehyde content was increased with increasing concentration of phenanthrene. F14 was higher cell surface hydrophobicity, and the cell surface hydrophobicities of F14 are respectively 69.7%, 65.2%. Exploring the changes of bacterial surface properties during the degrading progress is more deeply for investigating the degradation mechanism.