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几种常用ND HI试验方法的比较与分析
引用本文:卢玉葵,刘伟云,吴霞芳.几种常用ND HI试验方法的比较与分析[J].佛山科学技术学院学报(自然科学版),2001,19(2):75-77.
作者姓名:卢玉葵  刘伟云  吴霞芳
作者单位:佛山科学技术学院动物医学系,
摘    要:对几种常用ND HI试验方法作比较,结果表明,在全量法和微量法中以生理盐水为稀释液,以1%红细胞悬液作反应指示剂和100%凝集抑制为判定终点的方法效果为佳,全量法测得的HI滴度较微量法的约高0.5-1个滴度,其灵敏度和准确性较高,从而更适于实际工作中ND抗体水平的免疫监测。

关 键 词:鸡新城疫  红细胞凝集抑制试验  免疫监测
文章编号:1008-0171(2001)02-0075-03
修稿时间:2001年2月27日

Comparison and analysis of several different HI tests for ND
LU Yu kui,LIU Wei yun,WU Xia fang.Comparison and analysis of several different HI tests for ND[J].Journal of Foshan University(Natural Science Edition),2001,19(2):75-77.
Authors:LU Yu kui  LIU Wei yun  WU Xia fang
Abstract:After many times of tests for several different Hemagglutination-inhibition (HI) test, the result shows that the HI test with 1% chicken red blood cells as an indicator, physiological saline as dilutor and complete hemagglutination as determination point is the best choice among other methods. The one hundred percent inspection method is superior to micro determination method by a half or one titre, it means the former method will be suitable for the determination of the chicken level of ND in practical investigation.
Keywords:Newcastledisease(ND)  Hemagglutination  inhibition(HI)  Comparison
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