Selectable genetic markers for nematode transgenesis |
| |
Authors: | Rosina Giordano-Santini Denis Dupuy |
| |
Institution: | 1.Genome Regulation and Evolution, Inserm U869,Université de Bordeaux, Institut Européen de Chimie et Biologie (IECB),Pessac,France |
| |
Abstract: | The nematode Caenorhabditis elegans has been used to study genetics and development since the mid-1970s. Over the years, the arsenal of techniques employed in
this field has grown steadily in parallel with the number of researchers using this model. Since the introduction of C. elegans transgenesis, nearly 20 years ago, this system has been extensively used in areas such as rescue experiments, gene expression
studies, and protein localization. The completion of the C. elegans genome sequence paved the way for genome-wide studies requiring higher throughput and improved scalability than provided
by traditional genetic markers. The development of antibiotic selection systems for nematode transgenesis addresses these
requirements and opens the possibility to apply transgenesis to investigate biological functions in other nematode species
for which no genetic markers had been developed to date. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|