电磁刺激对成骨样细胞UMR-106 DNA合成的作用

为了从细胞水平研究低频电磁场影响成骨样细胞增殖的有效物理参数 ,并为其作用机制的解释提供依据 ,论文就几种类型电磁场对成骨样细胞 UMR- 10 6DNA合成的作用进行了一系列实验 ,通过 3 H-脱氧胸苷掺入检测 DNA合成的改变 ,发现特定频率、场强 (或磁感应强度 )组合的电磁场 (脉宽 0 .2 m s,10 V/cm,12 5 Hz附近的脉冲电场 ;1V/cm,10 Hz附近的交变电场 ;0 .5 m T,5 Hz附近的交变磁场 )能促进细胞 DNA合成水平显著提高 ,表明刺激骨细胞生长的电磁场无需复杂的波形。场强对 DNA合成有重要作用 ,只有在合适的场强范围内 ,一定频率的电磁场对细胞才有刺激作用。交变磁场所诱导的电场很微弱 ,表明磁场不只是通过诱导电场发挥作用的 ,研究磁场对细胞的作用应考虑包括磁场直接作用等全部可能的机制。

Effects of electromagnetic fields on DNA synthesis in an osteoblast-like cell line (UMR-106)

Effects of several types of low energy, low frequency electromagnetic fields (EMFs) on DNA synthesis in UMR 106 osteoblast like cells were investigated to explore the optimum physical parameters for stimulating cell proliferation and to provide a basis for determining its mechanism. DNA synthesis was judged using 3 H thymidine incorporation. The results showed that DNA synthesis in the cells increased with some specific combinations of the parameters (pulsed electric fields with pulse width 0.2ms , field strength 10V/cm, frequency 125Hz; sinusoidal electric fields with field strength 1V/cm,frequency 10 Hz; and sinusoidal magnetic fields with field density 0.5mT, frequency 5Hz). The results suggest that EMFs with some specific wave forms rather than complex forms can be used in clinical electrotherapy. In addition to frequency, field strength (or field density) within a suitable intensity played a significant role in causing DNA synthesis response. The energy of magnetically induced electric fields was so weak that cellular effects may not only be due to the induced electric fields. It is, therefore, important to consider all possible mechanisms including direct interaction between magnetic fields and cells.