| 光纤光谱方法快速检测尿液中的有机磷农药生物标志物 |
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| 引用本文： | 魏国芬,寇文杰,叶泰,王晶晶,肖虎勇,胡芹芹,艾永青,施少敏,周兴旺,吕鉴泉.光纤光谱方法快速检测尿液中的有机磷农药生物标志物[J].湖北师范学院学报(自然科学版),2011,31(3):60-64. |
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| 作者姓名： | 魏国芬 寇文杰 叶泰 王晶晶 肖虎勇 胡芹芹 艾永青 施少敏 周兴旺 吕鉴泉 |
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| 作者单位： | 1. 湖北师范学院污染物分析与资源化技术湖北省重点实验室,湖北黄石,435002
2. 黄石市中心血站,湖北黄石,435000 |
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| 摘 要： | 磷酸二甲酯和磷酸二乙酯是典型的有机磷农药代谢型生物标志物。基于二烷基磷酸酯与二苯基重氮甲烷的作用,建立了一种用光纤光谱方法快速检测有机磷农药代谢型生物标志物的新方法。分别优化了反应介质、时间和温度等试验参数对该检测体系的影响。结果表明,在1.0μg/mL～40μg/mL范围内的磷酸二甲酯和磷酸二乙酯与光信号呈良好的线性关系,检出限(S/N=3)分别为0.25μg/mL和0.23μg/mL.方法具有操作简便,分析速度快和灵敏度高等特点,我们应用于尿液的分析。
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| 关 键 词： | 有机磷农药 生物标志物 光纤光谱 检测 |
Fiber-optical spectroscopy for rapid determinating biomarkers of organophosphorus pesticides in urine |
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| WEI Guo-fen,KOU wen-jie,YE Tai,WANG Jing-jing,XIAO Hu-yong,HU Qin-qin,AI Yong-qing,SHI Shao-min,ZHOU Xing-wang,LV Jian-quan.Fiber-optical spectroscopy for rapid determinating biomarkers of organophosphorus pesticides in urine[J].Journal of Hubei Normal University(Natural Science),2011,31(3):60-64. |
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| Authors: | WEI Guo-fen KOU wen-jie YE Tai WANG Jing-jing XIAO Hu-yong HU Qin-qin AI Yong-qing SHI Shao-min ZHOU Xing-wang LV Jian-quan |
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| Institution: | WEI Guo-fen1,KOU wen-jie2,YE Tai1,WANG Jing-jing1,XIAO Hu-yong1,HU Qin-qin1,AI Yong-qing1,SHI Shao-min1,ZHOU Xing-wang1,LV Jian-quan1(1.Hubei Key Laboratory of Pollutant Analysis & Reuse Technology,Hubei Normal University,Huangshi 435002,China,2.Huangshi Blood Center,Huangshi 435000,China) |
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| Abstract: | Dimethylphosphate and Diethylphosphate are considered as typical biomarkers of organphosphorus pesticides.A new and fast method for the determination of dialkylphosphates has been developed by fiber-optical spectroscopy based on the color reaction with dialkylphosphates and diphenyl diazomethane.Reaction media,temperature and time of the derivatization were investigated.The results showed that the light signals are linearly with the concentration of DAP in the range from 1.0 to 40μg/mL.The detection limits（S/N=3） of dimethylphosphate and diethylphosphate were 0.25μg/mL and 0.23μg/mL.This proposed method was successfully applied in urine with the character of easy,sensitive and fast. |
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| Keywords: | organophosphorus pesticides biomarker fiber-optical spectroscopy detection |
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