RP-HPLC法测定人血草中黄连碱和白屈菜红碱的含量

目的：建立人血草干燥全草中黄连碱和白屈菜红碱含量的测定方法．方法：采用反相HPLC法．色谱柱为YMC—PackODS—AA（4．6mm×250mm，5肚m），以乙腈一水（0．2％磷酸＋0．2％三乙胺）体系梯度洗脱，检测波长280nm，流速1．0mL·min-1，柱温30℃．结果：黄连碱在13．24～264．8μg·mL叫质量浓度范围内面积与浓度呈良好的线性关系（r=0．9997）；加样回收率为99．4％，RSD为1．1％（n=6）．白屈菜红碱在6．36～127．2μg·mI．一质量浓度范围内面积与浓度呈良好的线性关系（r=0．9998）；加样回收率为101．6％，RSD为1．2％（n=6）．结论：本法简单、准确，重复性好，可用于人血草药材的质量．

Determination of Cptisine and Chelerythrine in Stylophorum Lasiocarpum (Oliv.) Fedde by RP-HPLC

This paper develops a method for determining of the content of coptisine and chelerythrine in Stylo- phorum lasiocarpum （Oliv.） Fedde. A HPLC method is established using YMC-Pack ODS-AA（4.6 mm X 250 mm, 5 t_tm） with the mobile phase acetonitrile and water （0.2 % phosphoric acid q- 0.2 % triethylamine） in gradient mode. The detection wavelength is set 280 nm. The flow rate is 1.0 mL ~ rain-~ and column temper- ature is set at 30~C. The linear relationship of coptisine is determined with the range from 13.24--264.8~g ~ mL-a （r=0. 999 7）. The average recovery as 99.4% with RSD 1.1%. The linear relationship of chelerythrine is determined with the range from 6.36--127.2/~g ~ mL-~ （r=0. 999 8）. The average recovery as 101.6% with RSD 1.2 %. The method is simple, accurate and reliable and can be used for quality control of Stylopho- rum lasiocarpum （Oliv.） Fedde.