| 毛细管电泳-柱端安培检测装置的研制 |
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| 引用本文： | 张兰,陈国南,方禹之.毛细管电泳-柱端安培检测装置的研制[J].福州大学学报(自然科学版),2000,28(6):85-88. |
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| 作者姓名： | 张兰 陈国南 方禹之 |
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| 作者单位： | 1. 福州大学化学系，福建福州 350002
2. 华东师范大学化学系，上海 200062 |
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| 基金项目： | 福州大学科技发展基金!(XKJYM 0 0 - 0 5 )的资助 |
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| 摘 要： | 设计了一种喷壁式毛细管电泳安培检测装置 .用原儿茶醛和原儿茶酚作为模型化合物考察了分离电压、电极与毛细管出口之间的距离对噪音和电极响应的影响 ,研究了清洗步骤对测定结果重视性的影响 .对该检测器的性能进行了测试 ,其分离效率可达 1.2× 10 5(原儿茶醛 )和1.1× 10 5(原儿茶酚 ) ,原儿茶醛和原儿茶酚检测限分别为 0 .2 5和 0 .50 μg/mL .
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| 关 键 词： | 毛细管电泳 安培检测 原儿茶醛 原儿茶酚 |
| 文章编号： | 1000-2243(2000)06-0085-04 |
| 修稿时间： | 2000年5月8日 |
An electrochemical cell for the capillary electrophoresis with end-column amperometric detection |
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| ZHANG Lan,CHEN Guo-nan,FANG Yu-zhi.An electrochemical cell for the capillary electrophoresis with end-column amperometric detection[J].Journal of Fuzhou University(Natural Science Edition),2000,28(6):85-88. |
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| Authors: | ZHANG Lan CHEN Guo-nan FANG Yu-zhi |
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| Institution: | ZHANG Lan 1,CHEN Guo-nan 1,FANG Yu-zhi 2 |
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| Abstract: | An electrochemical cell designed for the capillary zone electrophoresis with end-column wall-jet amperometric detection is reported. The three- electrodes system was used, with carbon-disc or copper-disc electrode as working electrode, Ag/AgCl electrode as reference electrode and platinum as auxiliary electrode. The distance between the working electrode and the capillary outlet on base-current and electrode respond were studied in detail. The investigation showed that good reproducibility could be obtained by using optimum wash-step. Application of this electrochemical cell for electrophoresis separation of the model compound protocatechuic aldehyde and protocatechuic acid has been studied, and high resolution and low detection limits have been obtained. |
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| Keywords: | capillary electrophoresis amperometric detection protocatechuic aldehyde protocatechuic acid |
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