北五味子木脂素对酒精性肝损伤小鼠免疫功能的影响

目的观察五味子木脂素对小鼠淋巴细胞亚群和自然杀伤细胞(NK细胞)的影响.方法应用五味子木脂素高、中、低3个剂量治疗酒精性肝损伤小鼠,联苯双酯对照,观察其对小鼠脏器系数、体液免疫功能、淋巴细胞增殖以及NK细胞活性的影响,利用流式细胞仪和MTT法检测其对淋巴细胞亚群、NK细胞的影响.结果五味子木脂素中剂量组胸腺、脾脏系数明显增加,与对照组比较差异具有统计学意义(P0.05);五味子木脂素中剂量组能显著降低CD8+水平,提高CD3+,CD4+水平及CD4+/CD8+比值,与对照组比较差异具有统计学意义(P0.05);五味子木脂素中剂量能提高NK细胞活性和T,B淋巴细胞增殖能力,与对照组比较差异具有统计学意义(P0.05).结论五味子木脂素能够增强酒精性肝脏损伤小鼠的免疫功能.

Effects of Schisandra Lignanoids on the Immune Function of Mice with Alcoholic Liver Injury

Objective To observe the effects of schisandra lignanoids( SCL) on the lymphocyte subgroup and NK cells in mice.Method High,medium and low doses of SCL were administrated to treat mice with alcoholic liver injury,bifendate pills as the control.Viscera coefficient of the mice was observed.Humoral immunity function, lymphocyte proliferation and activity of NK cells were tested by Spectrophotometer.The effects of SCL on lymphocyte subsets and NK cells were determined by flow cytometry instrument and MTT method.Results The thymus and spleen coefficients increased significantly in the medium group(P<0.05).Compared with the control group,CD8+ decreased obviously,CD3+,CD4+ and CD4+/CD8+ ratio significantly increased (P<0.05).Medium dose of SCL remarkably improved the activity of NK cells, and the proliferation ability of lymphocyte T and B (P<0.05).Conclusion SCL could enhance the immunity of the mice with alcoholic liver injury.