Immobilized subunits can function as an affinity sorbent to purify oligomeric enzymes: The usefulness of hybridization on the solid support |
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| Authors: | V I Muronetz L I Ashmarina N K Nagradova |
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| Institution: | (1) Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, 117234 Moscow, USSR |
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| Abstract: | Summary Immobilized dimers of yeast glyceraldehyde-3-phosphate dehydrogenase covalently bound to sepharose were shown to form hybrids with soluble dimers of the homologous enzymes present in crude tissue extracts (rat skeletal muscle, rat, rabbit and bovine hearts, rat liver, rat brain). Immobilized hybrid tetramers were then dissociated to form purified soluble enzymes. |
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