| 流速对蛋白质凝胶排阻层析分辨率的影响 |
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| 引用本文： | 原永洁,林贻箴.流速对蛋白质凝胶排阻层析分辨率的影响[J].山东师范大学学报(自然科学版),1995,10(1):75-77. |
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| 作者姓名： | 原永洁 林贻箴 |
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| 作者单位： | 山东师范大学生物学系 |
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| 摘 要： | 利用ＳｅｐｈａｄｅｘＧ－１００凝胶排阻层析进行蛋白质的分离，流速改变时，牛血清蛋白与卵清蛋白的分辨率亦随之变化，当流速达到最佳操作操作压下最大流速约１／４时，具有最高分辨率。
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| 关 键 词： | 凝胶排阻层析 分辨率 流速 蛋白质 分离 |
THE EFFECT OF FLOW RATE ON THE RESOLUTION OF GEL EXCLUSION CHROMATOGRAPHY FOR PROTEIN SEPERATION |
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| Yuan Yongjie, Lin Yizhen.THE EFFECT OF FLOW RATE ON THE RESOLUTION OF GEL EXCLUSION CHROMATOGRAPHY FOR PROTEIN SEPERATION[J].Journal of Shandong Normal University(Natural Science),1995,10(1):75-77. |
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| Authors: | Yuan Yongjie Lin Yizhen |
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| Abstract: | In this paper Sephadex G -100 exclusion chromatography was used for protein seperation .The resolution of bovine serum albumin to ovalbumin chan ged as flow tate varled. There was the highestresolution when flow rate reached one fourth of the maximum folw eate for G-100 at optimum operating pressure. |
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| Keywords: | Gel exclusion chromatography resolutionl flow rate |
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