齐墩果酸抑制肝癌细胞QGY增殖和诱导凋亡的作用研究

目的研究齐墩果酸对人肝癌细胞QGY增殖的作用及与细胞内钙离子浓度（Ca2＋]i）关系。方法将浓度分别为40、80、100μg／ml齐墩果酸作用肝癌H细胞QGY24h后，DAPI染色，以荧光显微镜观察细胞形态变化；以11组不同浓度齐墩果酸（5—400μg／ml）作用QGY细胞24h后，用四甲基偶氮唑蓝（Myr）法检测QGY增殖情况；分别以不同浓度齐墩果酸（80、100、120μg／ml）作用QGY细胞24h后，流式细胞仪检测细胞周期改变、细胞凋亡率和Ca2＋]i。结果细胞增殖被抑制并发生凋亡：不同浓度齐墩果酸能够抑制QGY细胞株增殖，且在5—120μg／mL范围内呈剂量依赖性，药物作用细胞24h、48的Ic50分别为76．27μg／mL和66．56μg／mL；处理组细胞周期在s期产生阻滞、细胞内Ca2＋]i较对照组显著增加，细胞凋亡率和Ca2＋]i与药物浓度存依赖关系。结论齐墩果酸能够抑制肝癌细胞QGY增殖和诱导其凋亡；诱导凋亡可能与细胞内Ca2＋]i增加有关。

Inhibition Proliferation and Induction Apoptosis Research of Oleanolic Acid on Hepatoma Cell Line QGY

Objective To investigate the role of oleanolic acid on the proliferation of human hepatoma Hep3B cell and study the relationship with intracellular Ca2 ＋ concentration （ Ca2＋ ] i）. Methods After QGY cells were treated with oleanolic acid of 40,80 and 100μg/ml respectively for 24 h and stained with DAPI, morphology changes of QJY cell were observed by fluorescmme microscopy. After QGY cells were treated with 11 group oleanolic acid of different concentrations ranged from 5 to 400μg/ml for 24 h, growth inhibition rates were measured by the MTr method. After QGY cells were treated with oleanolic acid of 80,100 and 120μg/ml respectively for 24 h, cell cycle, apoposis percentage and Ca2＋ ] i were checked by tlow eytometry. Results Ceil proliferation was inhibited. The apoptosis occurred. The proliferation of QJY cells could be inhibited by oleanolic acid of different concentrations in a dose dependent range from 5 to 120 μg/mL. After QJY cells were treated with OA for 24 h and 48 h,ICs0 was 76. 27 and 66.56μg/mL,respectively, Cell cycle was terminated at S phase by oleanolic acid. Intracellular Ca2＋ concentration was significantly increased. At the meantime, apoptosis ratios and increases of intracellular Ca2＋] i depended on the concentration of oleanolic acid. Conclusion Oleanolic acid may inhibit the growth of QJY cells and induce/be apoptosis by up-regulating intracellular calcium ion concentration.