诺迪康对AD模型大鼠学习记忆及海马AchE、ChAT活性的影响

目的研究诺迪康对阿尔茨海默病(AD)模型大鼠学习记忆及海马胆碱能系统的影响.方法将32只SD大鼠随机分成4组:模型组,安理中组,诺迪康组和正常组.采用双侧海马内注射Aβ-40制作大鼠AD模型,采用Morris水迷宫检测诺迪康对AD大鼠认知功能的影响;通过HE染色观察对AD大鼠的海马神经元形态的影响;采用免疫组化法观察对...

Effect of Nuodikang Capsule on Learning, Memory and Expression of ChAT and AchE in Hippocampus of AD Rats

Objective To explore the effect of Nuodikang capsule on learning, memory and activities of AchE and ChAT in hippocampus tissue of Alzheimer＇s disease （AD） animal model. Methods 32 rats were randomly divided into four groups：model group, Aricept Tablets group, Noodikang capsule group and normal group. The amyloid β1-40 was injected into the bilateral hlppocampus to form AD model in rats, Morris water maze testing system was adopted to observe the changes of rats＇. The appearance of AD rats hippocampal neurons were observed by HE staining. The activities of AchE and ChAT were detected by immunohistochemisty. The changes of AchE and ChAT mRNA were detected by RT-PCR. Results The escape latency of the place navigation in Aricept Tablets group, Nuodikang capsule group and normal group were significantly shorler than which in the model group（ P 〈 0. 05 ）. The average optical density of positive Ache cells significantly reduced. Compared to model group（ P 〈 0.05 ）, the average optical density of positive ChAT cells significantly increased in the other three groups. The level of AchE mRNA significantly reduced. Compared with model group（ P 〈 0.05 ）, the level of ChAT mRNA significantly increased in the other three groups. Conclusion Nuodikang capsule can improve the ability of learning and memory in AD rats. The mechanism maybe is related to CHAT, AchE.