基于颜色特征的酶标板孔菌液浑浊度与菌体生长情况研究

为了快速检测酶标板培养孔菌体不同时期的生长情况,代替传统的微生物生长中人工对菌体数量的测得从而对其生长情况判断的方法;通过对JM09菌株在加有LB液体培养基的酶标板中连续24 h的培养,采用机器视觉的图像采集和图像处理算法对酶标板孔培养孔的菌体颜色特征的每隔一个小时进行颜色特征值计算方法;得到了菌体不同生长时期菌液浑浊度和颜色特征值的曲线变化关系;提出一种根据菌体培养时菌液浑浊度变化和颜色特征值的关系对菌体数量及生长情况判断的方法.

Image Detection Method for Growth Status of Microbiota in ELISA Plate Hole

In order to quickly detect the growth of the cells in different time periods,the method of judging the growth of the number of cells in the traditional microbial growth was used to judge the growth of the cells.The JM09 strain was added with LB liquid.The culture medium was cultured for 24 hours in a continuous manner,and the color eigenvalues were calculated every hour of the bacterial color characteristics of the wells of the microplates by machine vision image acquisition and image processing algorithms;The relationship between the turbidity and color characteristic values of bacterial liquid in different growth stages was proposed.A method for judging the number and growth of bacterial cells based on the relationship between the turbidity change and the color characteristic value of the bacterial culture was proposed.