| 不同碳源及培养方式对小麦花药培养愈伤组织诱导率的影响 |
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| 引用本文： | 高书国,毕艳娟,乔亚科. 不同碳源及培养方式对小麦花药培养愈伤组织诱导率的影响[J]. 河北科技师范学院学报, 1992, 0(3) |
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| 作者姓名： | 高书国 毕艳娟 乔亚科 |
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| 作者单位： | 河北农业技术师范学院农学系，河北农业技术师范学院农学系，河北农业技术师范学院农学系 |
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| 摘 要： | 以5个小麦杂交组合F_1为材料,采用两种培养方式,在不同碳源的三种培养基上进行花药培养。结果表明,用麦芽糖取代常用培养基中的蔗糖,可有效地提高愈伤组织的诱导率,提早出愈时间,用葡萄糖代替蔗糖则具有不利影响;固体培养和固体+液体培养两种方式比较,愈伤组织诱导率没有明显差异。
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| 关 键 词： | 小麦 花药培养 碳源 培养方式 |
EFFECT OF DIFFERFNT CARBON SOURCES AND CULTURAL METHODS ON CALLUS INDUCTION RATIO OF ANTHER CULTURE IN WHEAT |
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| Gao Shuguo Bi Yanjuan Qiao Yake. EFFECT OF DIFFERFNT CARBON SOURCES AND CULTURAL METHODS ON CALLUS INDUCTION RATIO OF ANTHER CULTURE IN WHEAT[J]. Journal of Hebei Normal University of Science & Technology, 1992, 0(3) |
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| Authors: | Gao Shuguo Bi Yanjuan Qiao Yake |
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| Abstract: | The anthers of five wheat hybrids F_1 cultured on three C_(17) media containing different carbon souces and two cultural methods were studied. Results indicated that callus induction ratio was raised availably and callus production time was shorter when the sucrose was replaced by malt sugar in conventional media. When the sucrose was replaced by glucose the effects were adverse. There was no significant difference of the induction ratio when solid+liguid culture was compared with solid culture. |
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| Keywords: | wheat anther culture carbon source cultural method |
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