| 细胞粘附肽Arg-Gly-Asp的合成与生物活性检测 |
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| 引用本文： | 王巍,王丽萍,李然,石玉红. 细胞粘附肽Arg-Gly-Asp的合成与生物活性检测[J]. 长春大学学报, 2006, 0(6) |
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| 作者姓名： | 王巍 王丽萍 李然 石玉红 |
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| 作者单位： | 长春工业大学化工学院 吉林长春130012(王巍，李然)，吉林大学基础医学院药理教研室 吉林长春130022(王丽萍)，朝阳师范高等专科学校生化系 辽宁朝阳122000(石玉红) |
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| 摘 要： | 采用固相多肽合成法合成Ag-G ly-Asp(RGD)三肽,以天门冬氨酸计RGD三肽收率为75%,采用MTT法进行合成肽RGD抑制人纤维肉瘤细胞HT1080转移机理方面的研究,证实了人工合成的RGD肽能抑制人纤维肉瘤细胞HT1080与纤维连接蛋白(FN)的粘附。
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| 关 键 词： | Arg-Gly-Asp 多肽 固相合成法 粘附 |
The synthesis of cell adhesion peptide Arg-Gly-Asp and biology activity examination |
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| WANG Wei,WANG Li-ping,LI Ran,SHI Yu-hong. The synthesis of cell adhesion peptide Arg-Gly-Asp and biology activity examination[J]. Journal of Changchun University, 2006, 0(6) |
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| Authors: | WANG Wei WANG Li-ping LI Ran SHI Yu-hong |
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| Affiliation: | WANG Wei~1,WANG Li-ping~2,LI Ran~1,SHI Yu-hong~3 |
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| Abstract: | Solid phase method is used to synthesize RGD in this paper,the yield is 75?lculated from the first amino acid anchored resin.The mechanism of synthesized peptide RGD on the metastasis of human fibrosarcoma cell HT 1080 is studied by MTT method,It shows that the synthesized polypeptide RGD can inhibit the adhesion between HT1080 and FN. |
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| Keywords: | Arg-Gly-Asp polypeptide solid phase method adhesion |
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