The apoptosis and cell cycle changes of SUD4 and DOHH2 induced by topotecan |
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| Authors: | Feng Xiaorong Zheng Junying Deng Fengjiao Paul Smith Cai Kun |
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| Affiliation: | (1) Unit of Molecular Biology, Israel National Institute of Oceanography, Tel Shikmona, P. O. B. 8030, 31080 Haifa, Israel;(2) College of Life Sciences, Wuhan University, 430072 Wuhan, China;(3) Department of Pathology, College of Medicine University of Wales, CF4 4XN Cardiff, UK |
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| Abstract: | Topotecan (TPT), a semisynthetic analogue of the natural product camptothecin is a cell cycle-specific drug with antitumor activity. To clarify the effect of TPT on SUD4 and DOHH2 cell line in this study, we examined the apoptosis and cell cycle changes of the two human cancer cell lines by exposing to TPT for 18 hours at various concentrations. The linear relationship between apoptosis cell number and the concentration of TPT was observed by means of Flow Cytometry and Annexin V assay. Then, DOHH2 cell is much more sensitive to TPT than SUD4 cell. In addition, Cell Question Software Assay showed positive relationship between the frequency of cells accumulated in S-phase and the concentration of TPT. The least concentration of TPT to change cell cycle is 5 nmol·L−1 in both cell lines. These results suggest that the inducing apoptosis of cancer cells is one of mechanism of TPT antitumor activity. Feng Xiaorong: born in 1965, Post-doctoral Research Fellow, Engaging in Cell and Molecular Biology. |
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| Keywords: | Topotecan (TPT) flow cytometry apoptosis cell cycle SUD4 and DOHH2 |
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