| Effects of Cholate on the Stability of Bacteriorhodopsin in Alkaline Solution |
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| 引用本文： | 王居硕,许强,赵南明. Effects of Cholate on the Stability of Bacteriorhodopsin in Alkaline Solution[J]. 清华大学学报, 1997, 0(1) |
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| 作者姓名： | 王居硕 许强 赵南明 |
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| 作者单位： | Wang Jushuo，Xu Qiang，Zhao Nanming ** Department of Biological Science and Biotechnology，State Key Laboratory of Biomembrane and Membrane Biotechnology，Tsinghua University，Beijing 100084 |
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| 摘 要： | ＥｆｆｅｃｔｓｏｆＣｈｏｌａｔｅｏｎｔｈｅＳｔａｂｉｌｉｔｙｏｆＢａｃｔｅｒｉｏｒｈｏｄｏｐｓｉｎｉｎＡｌｋａｌｉｎｅＳｏｌｕｔｉｏｎ＊ＷａｎｇＪｕｓｈｕｏ（王居硕），ＸｕＱｉａｎｇ（许强），ＺｈａｏＮａｎｍｉｎｇ（赵南明）＊＊Ｄｅｐａｒｔｍｅｎｔｏ...
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Effects of Cholate on the Stability of Bacteriorhodopsin in Alkaline Solution * |
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| Wang Jushuo,Xu Qiang,Zhao Nanming. Effects of Cholate on the Stability of Bacteriorhodopsin in Alkaline Solution *[J]. Tsinghua Science and Technology, 1997, 0(1) |
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| Authors: | Wang Jushuo Xu Qiang Zhao Nanming |
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| Affiliation: | Wang Jushuo,Xu Qiang,Zhao Nanming ** Department of Biological Science and Biotechnology,State Key Laboratory of Biomembrane and Membrane Biotechnology,Tsinghua University,Beijing 100084 |
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| Abstract: | The effects of cholate on the structure of bacteriorhodopsin (bR) in the purple membrane and the effects of alkali on native and cholate treated bR were studied using absorption spectra, fluorescence spectra and CD spectra. Treatment mass fraction of 10% cholate at room temperature for 48 h had no obvious effect on the structure of bR, yet changed the composition of the purple membrane, making cholate treated bR more stable in alkaline solution than native bR. |
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| Keywords: | bacteriorhodopsin (bR) purple membrane cholate absorption spectra fluorescence spectra CD spectra |
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