G-CSF对亚急性脑缺血老龄鼠学习记忆改善及胶质细胞可塑性的影响

目的:研究粒细胞集落刺激因子(G-CSF)对亚急性脑缺血老龄鼠认知障碍改善及大脑海马胶质细胞可塑性的影响,并分析认知改善与胶质细胞可塑性的相关性.方法:14月龄雄性SD大鼠30只,随机分为2VO/NS组、Sham/NS组、2VO/G-CSF组,每组10只.利用Morris水迷宫实验评估大鼠学习记忆功能,利用免疫组化及图像分析技术检测海马CA1区的胶质细胞数目及其胞质突起长度.结果:水迷宫实验:2VO/NS组大鼠逃逸时间长于Sham/NS组(P<0.01)和2VO/G-CSF组(P<0.05);2VO/NS组大鼠停留于平台所在象限的时间少于Sham/NS(P<0.01)和2VO/G-CSF组(P<0.05);2VO/NS组大鼠跨过平台区域的次数少于Sham/NS和2VO/G-CSF组(P<0.01).免疫组化染色:2VO/NS组海马CA1区GFAP阳性细胞较Sham/NS组和2VO/G-CSF组减少(P<0.05).2VO/NS组大鼠GFAP阳性细胞胞质突起长度低于Sham/NS组和2VO/G-CSF组(P<0.05).认知功能与胶质细胞可塑性的相关性分析:2VO/NS组、Sham/NS及2VO/G-CSF组海马CA1区胶质细胞的数量、胞质突起长度的变化与空间记忆功能改善均呈正相关.结论:G-CSF可有效改善亚急性脑缺血老龄鼠的认知障碍,而其认知障碍与G-CSF干预诱导的可塑性改变密切相关.

Effects of G-CSF on Cognitive Handicap and Gliacyte Plasticity after Subacute Cerebral Hypoperfusion in Aged Rats

Objective：To investigate the effects of G-CSF on gliacyte plasticity and cognitive handicap, and analyze their correlation after subacute cerebral hypoperfusion in aged rats. Methods： 30 male-SD-fourteen-month rats were randomly divided into 2VO/NS group, Sham/NS group and 2VO/G-CSF group, each group has 10 rats. Learning and memory function was accessed by Morris water maze test, the number and cytoplasmic process length of GFAP positive cell in hippocampal CA1 region were analyzed by immunohistochemistry and image analysis. Results： Escape latency of 2VO/NS group was longer than Sham/NS （P 0.01） and 2VO/G-CSF （P0.05）. 2VO/NS group spent less time in the platform quadrant than Sham/NS （P0.01） and 2VO/G-CSF （P0.05）. As for time of crossing the platform area, 2VO/NS group was less than Sham/NS and 2VO/G-CSF （P0.01）. The number of GFAP positive cell in hippocampal CA1 region of 2VO/NS group was significant less than Sham/NS and 2VO/G-CSF （P0.05）. The cytoplasmic process length of GFAP positive cell of 2VO/NS group was significant shorter than Sham/NS and 2VO/G-CSF （P0.05）. For 2VO/NS, Sham/NS and 2VO/GSF group, the number and cytoplasmic process length of glia cells in hippocampal CA1 region were positive related to spatial memory.Conclusion：After subacute cerebral hypoperfusion, the function of spatial learning and memory were impaired in aged rats, GCSF can enhance congnitive function, promote alteration of gliacyte plasticity. Simultaneously, the alteration of gliacyte plasticity related to improvement of spatial memory.