应用层流柜饲养裸鼠的体会

裸鼠是先天性胸腺缺陷动物 ,T细胞缺乏 ,是现今进行人癌异种移植的理想动物。然而由于裸鼠的饲养条件要求较高 ,使其应用受到了很多的限制。我们教研室经过几年的探索 ,在无菌实验室内使用层流柜 ,再加上比较严格的管理制度 ,成功地饲养裸鼠 18批次 ,5 6 0余只 ,都顺利地完成了实验。所以 ,在实验室条件下使用层流柜短期饲养裸鼠是可行的

Experience of Breeding Nude Mice in Laminar Flow Cabinet

Aim To summarize the Experience of breeding nude mice in laminar flow cabinet.Methods\ 2 sets of JD\|25 laminar flow cabinet were used and maintained according to the instruction manual provided by the producer.560 nude mice in 18 groups were bred in the laminar flow cabinets in a separated clean room for the total time of 6 years.The room was disinfected every day and the lights were on for 10 hours each day.Fallout bacteria were detected every month.Results\ The room temperature was kept 22℃—28℃,humidity 40%—60%,bacteria colony number was 3/dish on average.The ammonia concentration in the air of the room and cabinets was lower than 10ppm,positive pressure was kept in the cabinets,the noise in the room was below 55dB.All the nude mice bred in the cabinets were well enough for the experimental studies.Conclusion\ Laminar flow cabinet used in a clean room is safe and easy way to breed nude mice.