小动物活体生物发光成像技术在肿瘤转移研究中的应用

建立小动物活体生物发光成像技术,研究三氧化二砷(As2O3)抑制小鼠B16恶性黑色素瘤的肺转移作用的方法,比较活体生物发光成像技术与常规动物实验技术的优劣性;并探讨As2O3抗小鼠恶性黑色素瘤肺转移的可能机制。结果表明,活体生物发光成像技术可以非侵袭性地动态监测黑色素瘤B16细胞在小鼠肺部的转移过程以及评估As2O3的体内抑制B16肺转移的作用,且活体生物发光成像技术的检测灵敏度优于传统肺转移瘤结节计数技术;As2O3主要通过减低肿瘤新生血管形成、促使肿瘤组织坏死而发挥抗小鼠黑色素瘤肺转移的作用。

Application of in vivo bioluminescence imaging on the study of tumour metastasis

The goal of this study was to establish the technology ofmonitoring the effects of arsenic tri- oxide on metastasis of murine malignant melanoma with in vivo bioluminescence imaging technique, and compare the superiority and disadvantage of in vivo bioluminescence imaging and conventional ani- mal experimental methods, and the anti- metastatic mechanism of arsenic trioxide was discussed. The results showed that the in vivo bioluminescence imaging could successfully be used to monitor noninva- sively and dynamically the process of the pulmonary metastasis of B16 melanoma cells and evaluate the inhibition efficacy of arsenic trioxide on the pulmonary metastasis of murine B16 melanoma cells, and compared with conventional pulmonary metastatic nodule count, the in vivo bioluminescence imaging possessed the advantages of non-invasion, real-time, dynamic visualization and hypersensitivity. Ar- senic trioxide exerted its significant anti-pulmonary metastasis effects of murine melanoma via repress- ing neovascularization of metastatic tumor to facilitate necrosis of the tumor cells.