水温对日本沼虾耗氧率及鳃组织CAT、SOD活力的影响

在pH 7.11±0.07,基准水温24.0±0.2℃条件下,采用静水停食法开展了水温对日本沼虾(体长4.29±0.32 cm,体质量1.80±0.12 g)存活、耗氧率及鳃组织CAT、SOD酶活力的影响实验。结果表明(:1)48 h内水温12.5℃和32.5℃实验组日本沼虾的死亡率均超过50%,而15～30℃范围内的存活率则为100%(;2)水温22.5～27.5℃范围内,日本沼虾昼均、夜均及各观测时段耗氧率均随水温升高而增大(P<0.05),但耗氧高峰时段和耗氧昼夜节律均未发生改变;(3)48 h内,水温22.5℃和25℃实验组日本沼虾鳃组织SOD活力时序变化步调一致且无差异(P<0.05),各时段观测值均低于27.5℃实验组(P<0.05),低谷期较27.5℃实验组后延12 h;(4)水温22.5～27.5℃日本沼虾鳃组织CAT活力在48 h内均较为稳定,且均随水温升高而显著增大(P<0.05)。

The Effect of Consumption Rate and CAT SOD Enzyme Activity From Water Temperature on Macrobrachium mipponensis

The thesis carried out the research about the effect of survival rate,consumption rate and CAT,SOD enzyme activity of gill tissue from water temperature on Macrobrachium mipponensis(body lenth 0.32 cm,body weigt 1.80±0.12 g),on condition that pH 7.11±0.07,benchmark water temperature at 24.0±0.2 ℃,in stagnant watehr and no feeding.The result showed that:(1) the death rate exceeded 50% in the groups when water temperature were 12.5 ℃ and 32.5 ℃,yet the death rate was 100% in the groups which water temperature were 15-30 ℃ within 48 h on M.mipponensis;(2) the consumption rate of daily average,and night average both amplified with water temperature raised during 22.5-27.5 ℃ water temperature,yet neither peak period nor rhythm of consumption had changes;(3) the change rhythm of SOD enzyme activity showed synchronous and no difference between 22.5 ℃ group and 25 ℃ group,and both under 27.5 ℃ group every observing time interval(P<0.05),low peak period of 22.5 ℃ group and 25 ℃ group delay 12 h compared with 27.5 ℃ group.(4) CAT enzyme activity of gill tissue showed unconverted,and amplified with water temperature rising significantly,during 22.5-27.5 ℃ water temperature within 48 h about M.mipponensis.