| 液体悬浮培养诱导金钗石斛原球茎增殖的研究 |
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| 引用本文： | 张宇斌,乙引,陈训,罗天霞,王鸿,游路,肖泓邻.液体悬浮培养诱导金钗石斛原球茎增殖的研究[J].贵州师范大学学报(自然科学版),2012,30(6):8-11. |
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| 作者姓名： | 张宇斌 乙引 陈训 罗天霞 王鸿 游路 肖泓邻 |
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| 作者单位： | 1. 贵州师范大学生命科学学院,贵州贵阳,550001
2. 贵州省科技厅,贵州贵阳,550001 |
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| 基金项目： | 贵州省青年教师基金项目 |
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| 摘 要： | 以金钗石斛原球茎为实验材料,通过液体悬浮培养,研究金钗石斛原球茎的增殖和多糖成份积累进行了研究。结果表明:金钗石斛在B5培养基+1.0mg.L-1NAA,蔗糖浓度为3%,10%香蕉提取物上清液的液体培养基中增殖最佳,可溶性多糖成分累积相对较高。
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| 关 键 词： | 金钗石斛 原球茎增殖 悬浮培养 |
Study on the proliferation of protocorm in Dendrobium nobile by fluid suspension culture |
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| ZHANG Yu-bin,YI Yin,CHEN Xun,LUO Tian-xia,WANG Hong,YOU Lu,XIAO Hong-lin.Study on the proliferation of protocorm in Dendrobium nobile by fluid suspension culture[J].Journal of Guizhou Normal University(Natural Sciences),2012,30(6):8-11. |
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| Authors: | ZHANG Yu-bin YI Yin CHEN Xun LUO Tian-xia WANG Hong YOU Lu XIAO Hong-lin |
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| Institution: | 1(1.School of Life Science,Guizhou Normal University,Guiyang,Guizhou 550001,China; 2.Guizhou Science and Technology Department,Guiyang,Guizhou 550001,China) |
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| Abstract: | In this paper,we performed a research based on the experimental material of dendrobium nobile protocorm through liquid suspension culture to study the dendrobium nobile protocorm multiplication and the polysaccharide accumulation.The result showed that: dendrobium nobile had the best proliferation in the liquid culture which mixed of B5 medium plus 1.0mg·L-1 NAA,3% sucrose concentration and 10% banana extract supernatant.Soluble polysaccharide accumulation is much more as well. |
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| Keywords: | Dendrobium nobile protocorm multiplication suspension culture |
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