BMSCs过表达BMP-4复合同种异体骨修复软骨缺损

目的 针对关节软骨损伤后修复困难,采用腺病毒(Ad)载体介导骨形态发生蛋白(BMP-4)修饰骨髓间充质干细胞(BMSCs),并复合同种异体骨软骨柱支架,以期实现关节软骨有效再生.方法 制备同种异体骨软骨柱,BMSCs过表达BMP-4复合同种异体骨修复关节软骨损伤,膝关节标本取材大体观察及分析,HE、番红固绿、Masso...

Repairment of Cartilage Defects through BMSCs Overexpression BMP-4 with Allogeneic Bone

Objective In our research,we used BMSCs modified by BMP-4 through adenovirus and combined with allogeneic bone scaffold in order to have an effective regeneration of articular cartilage. Method Firstly,manufacturing the allogeneic bone cartilage column grafts,then repairing the articular cartilage defects with allogeneic bone cartilage column grafts which are modified by BMSCs overexpressing BMP-4. After that,taking a general observation and analysis of knee joint specimens. Lastly,taking a quantitative analysis of the regeneration of articular cartilage through the HE,saffron O,Masson and immunohistochemistry. Result The result of the HE,safranine O,Masson and immunohistochemistry indicated that the experimental group could significantly increase the content of cartilage fibers in the regeneration of articular cartilage. Conclusion The composite of allogeneic bone scaffolds with BMSCs overexpressing BMP-4 can significantly promote the regeneration of cartilage cartilage and the repairment of cartilage damage.