孕期营养补充剂对妊娠母鼠和仔鼠行为学影响

目的 观察孕期添加多种维生素和矿物质营养补充剂对母鼠和仔鼠自主活动与探究行为的影响,以及对仔鼠发育的影响.方法 SPF级昆明小鼠交配见栓后随机分为低剂量组、中剂量组、高剂量组和溶剂对照组,孕期添加营养补充剂(孕早期、孕中期、孕晚期3个阶段的产品)按人体推荐日摄入量2 g/d换算小鼠的等效剂量,以等效剂量的0.5倍、1倍...

Effect of Nutritional Supplements during Pregnancy on Spontaneous Motor Activity and Explorative Behavior of Mother and Filial Mice

Objective To observe the effect of adding multiple vitamins and mineral nutritional supplements during pregnancy on the autonomy and exploration behavior of mother and filial mice rats,as well as,on the development of filial mice. Method SPF Kunming mice were randomly divided into low-dose group,medium-dose group,high-dose group,and vehicle control group after mating and seeing the suppository. The nutritional supplements added during pregnancy were converted into mice according to the recommended daily intake of 2 g/d. Comparing to the solvents as the control group,the experimental group divided into 3 groups,whose effective dose were 150 mg/( kg·d),300 mg/( kg·d) and 600 mg/( kg·d),respectively. Observing the birth weight,weaning weight and weaning weight index of 1 week after weaning,at the same time,we conduct the open field experiment on the mother and filial mice 1 week after weaning to observe their behavioral differences. Result The birth weight of the3 dose groups increased compared with the control group,and the difference between the high dose group and the control group was statistically significant( P<0. 05). The body weight of the filial mice on the day of weaning was significantly higher than that of the control group( P<0. 05,P<0. 01). One week after weaning,the weight of the 3 dose groups increased significantly compared with the control group( P<0. 01). There was no significant changes in the 2 analysis indexes of horizontal and vertical movements of postpartum mother rats( P > 0. 05),and the 2 analysis indexes of the staying time in the central area and the numbers of crossing the central area showed an increasing trend. One week after weaning,there was no significant difference between the horizontal and vertical movement indexes of female filial mice( P > 0. 05),but the horizontal movement indexes showed an increase of5. 16% and 14. 79% in the medium and high dose groups;in the medium and high dose groups,the residence time of the central district increased by 4. 59% and 72. 95% respectively, and the high-dose group increased significantly( P<0. 01);the number of crossings the central area increased by 8. 58% and 56. 89%,which the high-dose group increased significantly( P<0. 05). In male filial mice,3 experimental groups compared with the control group had an increase in the number of vertical exercise times,horizontal exercise time and horizontal exercise number of times. Among them,the difference in the number of vertical exercises between the high-dose group and the control group was statistically significant( P < 0. 05). Conclusion The addition of nutritional supplements to pregnant mothers during pregnancy can increase the body weight of the filial mice,which is benefit to the growth and development of the filial mice,and can increase their exploratory behavior without increasing the autonomous activity of the experimental animals.