产脂肪酶酵母的筛选及其18S rDNA序列分析

 从新疆石河子地区10分土样中,利用罗丹明B为指示剂,筛选到了90株有脂肪酶活性的酵母菌.滴定法测量野生菌株酶活,每毫升培养液酶活为1.1～6.6U,其中B9,C1,E7,F12等有成为生物催化剂的潜力.扩增了其中脂肪酶活性较高的9株的18SrDNA,测序后BLAST比对,并进行系统进化树分析,表明所得菌株分别与Candida,Rhodotorula,Trichosporon等属亲缘关系接近.进行部分生理生化试验,除H4,H8外,其它菌株结果与18SrDNA分析结论吻合.

The isolation of lipase-producing yeast and the sequence analysis of the18S rDNA genes

Using Rhodamine Bas indicator,90 lipase-producing yeasts were isolated from the soil samples of Shihezi in Xinjiang by plate isolation.Using titration to test the enzyme activity of the strains' culture fluid,titration was used with 1.1—6.6 Uper milliliter.It seemed that B9,C1,E7,F12 had the potentiality as the biocatalysts.The 18SrDNA was amplified from 9 strains which had a relatively higher lipase activity,after the sequencing,BLAST and phylogenetic analysis showed that they were close to Candida.,Rhodotorula.,Trichosporon..Some physiological and biochemical methods were used to prove that the results were congruent with the 18 srDNA analysis except H4 and H8.