油菜雄性不育系160S育性转换中保护酶活性变化

以温度敏感型甘蓝型油莱雄性不育系160S为试验材料,并以可育油菜31C为对照,对160S育性转换过程中叶片和花药保护性酶的活性等进行了研究,研究中测定了160S在育性转换温度敏感时期超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)等抗氧化酶活性以及丙二醛(MDA)含量....

Changes of Protective Enzyme Activities in Male Sterile Line 160S During Fertility Transformation

This research studied the activities of the protective enzyme in the leaves and anthers during the fertility transformation of the thermo-sensitive male-sterile line 160S of Brassica napus and selected the fertile rape as the contrast.The protective enzyme activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),ascorbate-peroxidase(APX) and malondialdehyde(MDA) content were measured.The results showed that the protective enzyme activities of 160S decreased and MDA content accumulated excessively during the fertility conversion,but the fertile line of 31C was of higher protective enzyme activity at the same temperature of the same period,while MDA content was relatively low and the anthers did not atrophy.Those differences of protective enzyme activity and MDA content accumulation were mainly occurred in the anthers of male sterile line 160S under natural temperature conditions.The results showed that one important factor of the fertility transformation of 160S probably was that anti-oxidation enzyme activities were abnormal which result in that the eliminating ability of reactive oxygen species decreased,and the reactive oxygen species and MDA accumulated excessively which probably destroyed the cell membrane.As a result,the pollens developed abnormally,then could not develop the pollen mother cell and behaved abortion traits eventually.