接骨木提取物有效成分含量与抑菌活性的关系

采用索氏提取法对接骨木叶片进行平行提取和顺序提取,并采用生长速率法测定了提取物对灰葡萄孢菌的抑菌活性,采用比色法测定了提取物中可溶性糖、可溶性蛋白、总黄酮和盐酸小檗碱的含量,以研究接骨木提取物有效成分含量与抑菌活性的关系,为接骨木活性物质的研究及更好的开发利用该属植物提供理论基础.结果表明,接骨木不同溶剂提取物的抑菌效果与以上四种有效成分的含量有关:提取物中可溶性糖或可溶性蛋白的含量越高,其对菌丝生长的促进作用越强,总黄酮或盐酸小檗碱的含量越高,其对菌丝生长的抑制作用越强.

The Study on the Relationship between Active Substance of Sambucus williamsii Hance and Antibacterial Activity

The soxhlet extraction method for extracting Sambucus williamsii Hance leaves were by parallel and order extraction.The effect of Sambucus williamsii Hance extracts derived on the hyphae growth of Botrytis cinerea Pers.Ex Fr.was studied using the growth rate method.And the extract that soluble sugar,soluble protein,total flavones and Beberine Hydrochloride were tested by colorimetry,in order to research the ralationship of effective constituents between the antibacterial active in S.williamsii Hance extract.The results have shown that the antibacterial activity have relate to extracts effective constituents with different solvents;the higher soluble sugar and soluble protein,the more powerful promoting activity to antibacteria effect.The higher total flavones and Beberine Hydrochloride,the more powerful antibacterial activity to anti-bacteria effect.This study can provide the theory basis for researching S.williamsii Hance active material,better exploiting and utilizing this genus plants.