| HPLC-ELSD法测定珠子参根茎中新化合物24(R)-珠子参苷R_1的质量比 |
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| 引用本文： | 王加付,彭绍,李兰杰,杨洁,孙婷,李绪文,金永日,时晓磊.HPLC-ELSD法测定珠子参根茎中新化合物24(R)-珠子参苷R_1的质量比[J].吉林大学学报(理学版),2014,52(5):1095-1099. |
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| 作者姓名： | 王加付 彭绍 李兰杰 杨洁 孙婷 李绪文 金永日 时晓磊 |
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| 作者单位： | 1. 吉林大学 化学学院, 长春 130012; 2. 黑龙江烟草有限责任公司, 哈尔滨 150001;3. 吉林大学 军需科技学院, 长春 130062 |
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| 基金项目： | 教育部博士学科点新教师基金 |
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| 摘 要： | 采用高效液相色谱蒸发光散射法(HPLC-ELSD)测定珠子参根茎中新化合物24(R)-珠子参苷R1的质量浓度.色谱条件为:Aglilent Zorbax SB-C18(ODS,4.6mm×250mm,5μm)色谱柱,流动相V(甲醇)∶V(水)=58∶42,流速1.0mL/min,柱温25℃,载气压力1.94×105 Pa,漂移管温度85℃,撞击器关闭,进样量20μL.结果表明:在0.40~20.0μg内,24(R)-珠子参苷R1线性关系较好,r=0.999 3(n=6);24(R)-珠子参苷R1的平均回收率为98.7%,RSD=1.28%(n=6).
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| 关 键 词： | 24（R）-珠子参苷R1 高效液相色谱蒸发光散射法 质量比 |
| 收稿时间： | 2013-10-24 |
Determination of New Compound 24(R)-Majoroside R1 in Panax japonicus C.A.Mey.var.Major(Burk.)C.Y.Wu et K.M.Feng by HPLC-ELSD |
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| WANG Jiafu,PENG Shao,LI Lanjie,YANG Jie,SUN Ting,LI Xuwen,JI Yongri,SHI Xiaolei.Determination of New Compound 24(R)-Majoroside R1 in Panax japonicus C.A.Mey.var.Major(Burk.)C.Y.Wu et K.M.Feng by HPLC-ELSD[J].Journal of Jilin University: Sci Ed,2014,52(5):1095-1099. |
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| Authors: | WANG Jiafu PENG Shao LI Lanjie YANG Jie SUN Ting LI Xuwen JI Yongri SHI Xiaolei |
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| Institution: | 1. College of Chemistry, Jilin University, Changchun 130012, China;2. Heilongjiang Tobacco Limited Liability Company, Harbin 150001, China;3. College of Quartermaster Technology, Jilin University, Changchun 130062, China |
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| Abstract: | The determination of the new compound of 24(R) majoroside R1 in Panax japonicus C.A. Mey. var. Major (Burk.KG-*3]) C.Y. Wu et
K. M. Feng was developed by HPLC\|ELSD method. The conditions of chromatography were Aglient Zorbax SB C18 Column (ODS, 4.6 mm×250 mm, 5 μm) was adopted; mobile phase was composed of V(methanol)∶V(water)=58∶42; flow rate was set at 1.0 mL/min; temperature of column was set at 25 ℃; carrier gas pressure was set at 1.94×105 Pa tube temperature of ELSD was set at 85 ℃; impactor was set in closed state. As the results show in the range of 0.4-20.0 μg, the linearity of 24(R) majoroside R1 is good, r=0.999 3(n=6), the average recovery was 98.7%, and RSD=1.28% (n=6). |
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| Keywords: | 24 (R)-majoroside R1 high performance liquid chromatography-evaporative light scatteringdatection (HPLC-ELSD) mass ratio |
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