烟草叶肉原生质体再生壁形成的电镜研究

电镜观察表明,新分离的烟草叶肉原生质体表面光滑,在质膜外没有纤维状物质,内质网和高尔基体几乎没有发育:培养1天后,线粒体明显增加,粗面内质网和高尔基体沿质膜发育,质膜变得粗糙;2天后,质膜发生折皱,高尔基囊泡向质膜外分泌它的内含物;6天后,质膜表面出现明显松散分布的再生壁。在培养基中附加200 ppm 香豆素处理,在培养1天后,质膜仍然光滑,未出现内质网和高尔基体;2天后,质膜开始变粗糙,内质网开始发育。试验表明,质膜、内质网、高尔基体在原生质体再生壁形成中起重要作用,并讨论了它们的可能作用。香豆索可能是在一定时同内,通过抑制质膜的活动和内质网、高尔基体的发育而抑制壁的再生。

Electron Microscopy Study On Cell Wall Regeneration In Tobacco Mesophyll Protoplasts

Freshly isolated and cultured tobacco(Nicotiana tabacum)mesophyll protoplasts were fixed with method of Fowke(1975),cutted into thin sections and observed by electron microscope.The surface of freshly isolated protoplasts was smooth and no fibrous subtances were observed on the outside of plasmalemma.Endoplasmic reti- culum(ER)and Golgi body poorly developed.After culturod 1 day,mitochondria distinctly increased,rough ER and Golgi body were developed on many sites closing to the plasmalemma,plasmalemma had become rough.After 2 days,plasmalemma shown fold,Golgi vesicles excreted their content to the surface of plasmalmalemma. After 6 days,showing on the electron micrograph the rather loosely reorganized regeneration cell wall.When protoplasts were cultured in the medium with 200 ppm coumarin,after 1 day,plasmalemma still smooth,ER and Golgi body not developed, after cultured 2 days,plasmalemma became rough,ER began to develop.Results show that plasmalemma,ER and Golgi body play an important role in the regenerative cell wall formation in protoplast,and coumarin inhibit cell wall regeneration by inhibition activation of plasmalemma and development of ER and Golgi body in certain time.