草履虫类核纤层蛋白的初步研究

本文以DGD包埋去包埋技术对草履虫的核纤层通过透射电镜和免疫荧光、分子杂交等技术进行了观察。结果显示，在核周存在由10nm纤维组成的核纤层；免疫荧光结果表明，在核周呈阳性反应，并在其表皮、口器等部位呈交叉的阳性反应；蛋白分子杂交的反应带在68kD处呈阳性反应。

A Preliminary Investigation on the Lamin-like Proteins in Paramecium

Paramecium caudatumwas first processed by DGD embedment and embedment-free technique and general technique for EM morphology. A perinuclear structure consisted of interacted filaments (we called lamina-like structure) was observed. Then using Western Blot assay, we found a lamin-like component band of 68kD protein in the three-step-fractionated cells. To investigate the distribution of the lamin-like protein in cells, a immunofluorescence experiment for in situ hybridization was designed using goat anti-lamin protein antibodies as the probes. The results revealed that the positive reactivity presented at different part of the cells. The perinuclear cross-actions were distinct, and cross-action with the oral apparatus and the cortex were also obtained.