| 选育具有青霉素抗性的青霉素高产菌种 |
| |
| 引用本文： | 王群,王世雯,付应艳,康自力,饶阳宾.选育具有青霉素抗性的青霉素高产菌种[J].河北省科学院学报,2006,23(1):74-76. |
| |
| 作者姓名： | 王群 王世雯 付应艳 康自力 饶阳宾 |
| |
| 作者单位： | 1. 华北制药股份有限公司菌种选育中心,河北,石家庄,050015
2. 华北制药股份有限公司102车间,河北,石家庄,050015 |
| |
| 摘 要： | 以青霉素产生菌87-55-21为出发菌株,筛选具有青霉素抗性的高产菌株,使用紫外线对孢子悬浮液照射110秒后,然后涂布在含有20000u/ml浓度青霉素的固体培养基上,分生孢子的死亡率为98.1%,处理后从176个菌落中筛选出87-58-6菌株,摇瓶发酵单位提高16.7%,87-58-5菌株投入生产后,平均发酵单位提高3.5%.
|
| 关 键 词： | 青霉素产生菌 紫外线 青霉素抗性 发酵单位 |
| 文章编号： | 1001-9383(2006)01-0074-03 |
| 修稿时间： | 2005年2月21日 |
The seed selection has the anti-penicillin of penicillin high produce the germ to grow |
| |
| WANG Qun,WANG Shi-weng,FU Ying-yan,KANG Zi-li,RAO Yang-bing.The seed selection has the anti-penicillin of penicillin high produce the germ to grow[J].Journal of The Hebei Academy of Sciences,2006,23(1):74-76. |
| |
| Authors: | WANG Qun WANG Shi-weng FU Ying-yan KANG Zi-li RAO Yang-bing |
| |
| Abstract: | A penicillin producing strain 87-55-21 was selected for penicillin resistance.The suspension of spore ir- radiated under ultraviolet ray for 110 second,then plated them on a medium containing penicillin(20000u/ml). After that,the death rate of conidiophore is 98.1%.After selected 176 colonies,we got the 87-58-6 strain.Under the condition of shaker-flask,the output was increased by 16.7%.After 87-58-6 strain is put into production,the average fermented output improves by 3.5%. |
| |
| Keywords: | Penicillin producing strain Ultraviolet ray Anti-penicillin Fermented output |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
