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选育具有青霉素抗性的青霉素高产菌种
引用本文:王群,王世雯,付应艳,康自力,饶阳宾.选育具有青霉素抗性的青霉素高产菌种[J].河北省科学院学报,2006,23(1):74-76.
作者姓名:王群  王世雯  付应艳  康自力  饶阳宾
作者单位:1. 华北制药股份有限公司菌种选育中心,河北,石家庄,050015
2. 华北制药股份有限公司102车间,河北,石家庄,050015
摘    要:以青霉素产生菌87-55-21为出发菌株,筛选具有青霉素抗性的高产菌株,使用紫外线对孢子悬浮液照射110秒后,然后涂布在含有20000u/ml浓度青霉素的固体培养基上,分生孢子的死亡率为98.1%,处理后从176个菌落中筛选出87-58-6菌株,摇瓶发酵单位提高16.7%,87-58-5菌株投入生产后,平均发酵单位提高3.5%.

关 键 词:青霉素产生菌  紫外线  青霉素抗性  发酵单位
文章编号:1001-9383(2006)01-0074-03
修稿时间:2005年2月21日

The seed selection has the anti-penicillin of penicillin high produce the germ to grow
WANG Qun,WANG Shi-weng,FU Ying-yan,KANG Zi-li,RAO Yang-bing.The seed selection has the anti-penicillin of penicillin high produce the germ to grow[J].Journal of The Hebei Academy of Sciences,2006,23(1):74-76.
Authors:WANG Qun  WANG Shi-weng  FU Ying-yan  KANG Zi-li  RAO Yang-bing
Abstract:A penicillin producing strain 87-55-21 was selected for penicillin resistance.The suspension of spore ir- radiated under ultraviolet ray for 110 second,then plated them on a medium containing penicillin(20000u/ml). After that,the death rate of conidiophore is 98.1%.After selected 176 colonies,we got the 87-58-6 strain.Under the condition of shaker-flask,the output was increased by 16.7%.After 87-58-6 strain is put into production,the average fermented output improves by 3.5%.
Keywords:Penicillin producing strain  Ultraviolet ray  Anti-penicillin  Fermented output
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