Molecular cloning and expression of interleukin Ibeta (IL-1β) from red seabream (Pagrus major) |
| |
作者姓名: | CAI Zhonghua SONG Linsheng GAO Chunping WU Longtao and QIU Lihua(1. Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China 2. Department of Fisheries Science Tianjin Agricultural College Tianjin 300381 China 3. The College of Life Science Ocean University of China Qingdao 266003 China) |
| |
作者单位: | 1. Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China; 2. Department of Fisheries Science,Tianjin Agricultural College,Tianjin 300381,China; 3. The College of Life Science,Ocean University of China,Qingdao 266003,China |
| |
基金项目: | Supported by the National High-Tech Project (Grant No. 2001AA628180),the Ministry of Science and Technology of China and a Post doctoral Scholarship (Grant No. 20011002110140 ) of K.C. WANG Education Foundation |
| |
摘 要: | The interleukin 1β (IL-1β) cDNA was cloned from the red seabream (Pagrus major) by homology cloning strategy. A cDNA fragment was amplified by PCR using two degenerated primers, which were designed according to the conserved regions of other known IL-1β sequences, and elongated by 3' ends and 5' ends RACE PCR to get the full length coding sequence of red seabream IL-1β (RS IL-1β). The sequence contained 1252 nucleotides that included a 5' untranslated region (UTR) of 84bp, a 3' UTR of 410 bp and an open reading frame (ORF) of 759 nucleotides which could be translated into a putative peptide of 253 amino acids with molecular weight of 28.6 kD and putative isoelectric point pI of 5.29. The deduced peptide contained two potential N-glycosylation sites and an identifiable IL1 family signature, but lacked the signal peptide and the clear ICE cut site, which were common in other nonmammalian IL-1β genes. The RS IL-1β had the highest homology with piscine IL-1β according to phylogenetic tree analysis.
|
本文献已被 CNKI 等数据库收录! |