| 他克莫司产生菌原生质体制备、再生与诱变 |
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| 引用本文： | 王晓蕾,高慧英,蒋沁,刘刚叁,张华,贺建功.他克莫司产生菌原生质体制备、再生与诱变[J].河北大学学报(自然科学版),2006,26(1):25-29. |
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| 作者姓名： | 王晓蕾 高慧英 蒋沁 刘刚叁 张华 贺建功 |
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| 作者单位： | 华北制药集团新药研究开发有限责任公司,微生物药物国家研究工程中心,河北,石家庄,050015 |
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| 基金项目： | 科技部科技基础条件平台建设计划 |
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| 摘 要： | 对筑波链霉菌的原生质体制备、再生条件进行了研究,建立了筑波链霉菌的原生质体诱变筛选体系.在此基础上,通过原生质体的紫外诱变处理,筛选得到1株高产菌株,与出发菌株相比,该菌株的平均发酶单位提高51.4%.通过原生质体的紫外诱变来筛选他克莫司高产菌株是一种行之有效的方法.
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| 关 键 词： | 他克莫司 筑波链霉菌 原生质体 紫外诱变 |
| 文章编号： | 1000-1565(2006)01-0025-05 |
| 修稿时间： | 2005年9月8日 |
Protoplasts Preparation, Regeneration and Mutagenesis of Streptomyces tsukubaensis |
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| WANG Xiao-lei,GAO Hui-ying,JIANG Qin,LIU Gang-san,ZHANG Hua,HE Jian-gong.Protoplasts Preparation, Regeneration and Mutagenesis of Streptomyces tsukubaensis[J].Journal of Hebei University (Natural Science Edition),2006,26(1):25-29. |
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| Authors: | WANG Xiao-lei GAO Hui-ying JIANG Qin LIU Gang-san ZHANG Hua HE Jian-gong |
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| Abstract: | Tacrolimus,a novel immunosuppressant isolated from Streptomyces tsukubaensis, has an impotant value of medical application.To screen high tacrolimus-producing mutants in S.tsukubaensis,the methods of protoplast preparation,regeneration and mutagenesis were established.Thereafter,the protoplasts of the original strain were treated by UV.Single colonies,which were formed by the regeneration of the UV-treated protoplasts,were selected to check their tacrolimus yield respectively.A high-yield mutant was obtained.The production of tacrolimus was increased 51.4% in comparison with the original strain.The result indicated that treating the protoplast of S.tsukubaensis with UV was a feasible and effective way to improve the production of tacrolimus. |
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| Keywords: | tacrolimus Streptomyces tsukubaensis protoplast ultraviolet mutation |
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