|
|||||
|
|
| 两种激发光诱导的硫氧还蛋白还原酶分子荧光光谱研究 | |
| 引用本文： | 杨涛,兰秀风,骆晓森,倪晓武,许琳.两种激发光诱导的硫氧还蛋白还原酶分子荧光光谱研究[J].南京理工大学学报(自然科学版),2005,29(1):55-58. |
| 作者姓名： | 杨涛 兰秀风 骆晓森 倪晓武 许琳 |
| 作者单位： | 1. 南京理工大学,理学院,江苏,南京,210094;第二军医大学,南京军医学院,江苏,南京,210099 2. 南京理工大学,理学院,江苏,南京,210094 3. 第二军医大学,南京军医学院,江苏,南京,210099 |
| 摘 要： | 分别以波长为253．7nm的紫外光及波长为407nm的紫色发光二极管为激发光源，诱导硫氧还蛋白还原酶(thioredoxin reductase，TR)分子产生荧光，用组合式多功能光栅光谱仪记录荧光谱。通过分析比较TR溶液与溶剂的荧光光谱，得到了这2种激发光诱导的TR分子荧光光谱的特性。该文的研究结果为进一步将荧光光谱法用于TR的定性、定量分析乃至结构分析提供了依据。 |
| 关 键 词： | 硫氧还蛋白还原酶 荧光光谱 激发波长 |
| 文章编号： | 1005-9830(2005)01-0055-04 |
| 修稿时间： | 2003年3月12日 |
Fluorescence Spectra of TR Molecule Induced by Lights with Different Wavelengths |
|
| YANG Tao,LAN Xiu-feng,LUO Xiao-sen,NI Xiao,XU Lin.Fluorescence Spectra of TR Molecule Induced by Lights with Different Wavelengths[J].Journal of Nanjing University of Science and Technology(Nature Science),2005,29(1):55-58. | |
| Authors: | YANG Tao LAN Xiu-feng LUO Xiao-sen NI Xiao XU Lin |
| Institution: | YANG Tao 1,2,LAN Xiu-feng1,LUO Xiao-sen1,NI Xiao-wu1,XU Lin2 |
| Abstract: | Multi-function grating spectrograph was used to measure the fluorescence spectra of the thioredoxin reductase (TR) excited by ultraviolet (UV) light of 253.7 nm and violet LED (Light Emitting Diode) light of 407 nm. The experimental results indicate that the TR solution can emit strong fluorescence induced by those two kinds of light. The characteristics of fluorescence from the TR molecule induced by UV light and LED light are obtained by analyzing the spectra of the TR solution and its solvent. The results can provide a foundation for better understanding of the TR structures by the method of fluorescence spectroscopy. |
| Keywords: | thioredoxin reductase (TR) fluorescence spectra induced wavelength |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |