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| TGEV截短S基因片段在多酵母表达系统中的表达 | |
| 引用本文： | 于天飞,黎明,吕建伟,闫冰,张建,孙天国,朱红标,孙一瑞.TGEV截短S基因片段在多酵母表达系统中的表达[J].高师理科学刊,2011,31(3):73-75. |
| 作者姓名： | 于天飞 黎明 吕建伟 闫冰 张建 孙天国 朱红标 孙一瑞 |
| 作者单位： | 1. 齐齐哈尔大学,生命科学与农林学院,黑龙江,齐齐哈尔,161006 2. 齐齐哈尔大学,计算机与控制工程学院,黑龙江,齐齐哈尔,161006 3. 南京农业大学动物医学院,江苏,南京,210095 |
| 基金项目： | 齐齐哈尔市科学技术计划项目，齐齐哈尔大学青年教师科研启动项目 |
| 摘 要： | 在多酵母表达系统中表达了含有猪传染性胃肠炎病毒S基因B和C抗原位点片段.经检测表明,不同类型酵母表达系统蛋白表达量和抗原性差异不大,研究中获得的重组蛋白为建立TGE血清学检测方法提供了必要的物质基础. |
| 关 键 词： | 猪传染性胃肠炎病毒 S基因 多酵母表达系统 表达 |
Expression of transmissible gastroenteritis virus truncated S gene in multiple yeast express systems |
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| YU Tian-fei,LI Ming,Lu Jian-wei,YAN Bing,ZHANG Jian,SUN Tian-guo,ZHU Hong-biao,SUN Yi-rui.Expression of transmissible gastroenteritis virus truncated S gene in multiple yeast express systems[J].Journal of Science of Teachers＇College and University,2011,31(3):73-75. | |
| Authors: | YU Tian-fei LI Ming Lu Jian-wei YAN Bing ZHANG Jian SUN Tian-guo ZHU Hong-biao SUN Yi-rui |
| Institution: | 1.School of Life Science and Agriculture and Forestry,Qiqihar University,Qiqihar 161006,China;2.School of Computer and Control Engineering,Qiqihar University,Qiqihar 161006,China;3.School of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China) |
| Abstract: | The B and C antigen sites of S gene were expressed in multiple yeast express systems.The quantity and antigenicity of expressed recombinant protein level in the three systems were almost the same.The recombinant protein in this research provided essential material foundation for establishment of TGE serology diagnostic method. |
| Keywords: | TGEV spike gene multiple yeast express systems expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |