| 免疫丙种球蛋白的缔合与分离及其动态性质 |
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| 引用本文： | 梁国眉 冯榕荫. 免疫丙种球蛋白的缔合与分离及其动态性质[J]. 中山大学学报(自然科学版), 1992, 31(1): 123-127 |
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| 作者姓名： | 梁国眉 冯榕荫 |
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| 作者单位： | 中山大学测试中心，中山大学测试中心，中山大学测试中心，中山大学测试中心 |
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| 基金项目： | 广州市科委重点研究资助项目 |
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| 摘 要： | 用凝胶渗透色谱法研究了聚乙烯基吡咯烷酮(PVP)、血清白蛋白和医用明胶等稳定剂对免疫丙种球蛋白(IgG)的作用和效果,结合准弹性激光光散射法研究了它们对IgG分子平移扩散系数和流体力学尺寸的影响。结果表明,PVP和血清白蛋白对IgG单体起着屏蔽稳定作用而防止其自身凝聚,但并不与IgG形成复合物,因而不会改变IgG单体的分子结构与构型。
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| 关 键 词： | 凝胶渗透色谱法 准弹性激光光散射 静脉注射免疫丙球蛋白 聚乙烯基吡咯烷酮 血清白蛋白 |
Association, Seperation and Dynamic Properties of Immunoglobulin G |
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| Liang Guomei,Feng Rongyin,Liang Xuewei,Lin Muliang Instrumentation Analysis and Research Center. Association, Seperation and Dynamic Properties of Immunoglobulin G[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 1992, 31(1): 123-127 |
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| Authors: | Liang Guomei Feng Rongyin Liang Xuewei Lin Muliang Instrumentation Analysis Research Center |
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| Affiliation: | Liang Guomei;Feng Rongyin;Liang Xuewei;Lin Muliang Instrumentation Analysis and Research Center |
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| Abstract: | The effects of several stabilizers on aggregation and dynamic properties of Immunoglobulin G (IgG) in aqueous solutions were studied by gel permeation chromatography and quasi-elastic laser light scattering. The results showed that both polyvinyl-pyrrolidone and human serum albumin were of protection and stabi-lization for IgG and restrained it from intramonomer association while did not form complexes with IgG; therefore. the addition of them did not change the structure and conformation of the IgG monomer. |
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| Keywords: | gel permeation chromatography quasi-elastic laser light scattering Immanoglobulin G for intravanous use polyvinyl-pyrrolidone human serum albumin |
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