一种简单方法提高原生质体再生植株频率

发展了一种简单有效的方法，提高从灿稻品种Ｐｕｓａ Ｂａｓｍａｔｉ１建立的超过１年龄悬浮细胞系中游离的原生质体再生植株频率。两步再生法，即先将愈伤组织转入含高浓度琼脂糖（１０ｇ／Ｌ）的再生培养基中培养后再转入含低浓度琼脂糖（４ｇ／Ｌ）的再生培养基中培养，有助于植株再生，两步再生法结合使用含高浓度激动素Ｋｉｎｅｔｉｎ（１０ｍｇ／Ｌ）的再生培养基，显著提高植株再生频率，利用此法，可使来源于超过１年龄的悬

Plant Regeneration from Protoplasts Was Efficiently Improved by A Simple Procedure

A simple and efficient method has been developed to improve plant regeneration from vrotoplasts isolated from over one-year old cell suspension culture of the Indica rice cv.Pusa Basmati 1.A two-step regeneration procedure,involoving the transfer of callus to shoot regeneration medium containing 10 g/L agarose prior to culture on a medium containing 4 g/L agarose,was found to improve plant regeneration.The two-step regeneration procedure,combined with a high concentration of kinetin(10.0 mg/L)in the medium,significantly increase plant regeneration.By this method,even though protoplasts were isolated from over one-year old cell suspension cultures,protoplast-derived plant regeneration frequency reached 16.1%,compared with less than 4% regeneration frequency without such treatment.These results give implication to use the similar protocol to improve pl ant regeneration from other plant species, especially recalcitrant species.