Effect of Nano red elemental selenium on GPx activity of broiler chick kidney cells<Emphasis Type="Italic">in vitro</Emphasis> |
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Authors: | Email author" target="_blank">Xu?Bao-huaEmail author Email author" target="_blank">Xu?Zi-rongEmail author Xia?Mei-sheng Hu?Cai-hong Deng?Yue-song Xiong?Li |
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Institution: | (1) College of Animal Science, Zhejiang University, 310029 Hangzhou, Zhejiang, China;(2) College of Animal Science, Zhandong Agricultural University, 271018 Taian, Shandong, China |
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Abstract: | A new selenium source, Nano red elemental selenium (Nano-Se) was used to study the effect on the GPx activity of broiler chick
kidney cells (BCKC)in vitro, Sodium selenite (Na2SeO3) and seleno-1-methionine (Se-Met) were used as the controls. The results showed that the effects of three kinds of Se forms
on the GPx activity of BCKC were accordant (p>0.05) compared with each other at 0.01. 0.05 and 0.10 μmol/L Se concentrations treatments. In the range of 0.00–0.10 μmol/L
Se concentrations, the GPx activity increased with elevation of Se concentrations in medium. For the three kinds of Se forms,
the GPx activity reached the climax at 0.10 μmol/L Se concentration. At 0.20 and 0.30 μmol/L Se concentrations, the influnces
of three kinds of Se forms were not accordant with one another. For Nano-Se, the GPx activity at 0.20 and 0.30 μmoi/L Se concentrations
remained the same as that at 0.10 μmol/L Se concentration treatment. For Se-Met, the GPx activity at 0.20 μmol/L Se concentration
treatment remained the same with 0.10 μmol/L treatment; the GPx activity at 0.30 μmol/L Se concentration treatment was declined
significantly (p<0.05) compared with 0.10 or 0.20 μmol/L treatment. For Na2SeO3, the GPx activity falled gradually with Se concentration increasing from 0.10 μmol/L to 0.30 μmol/L, and at 0.30 μmol/L Se
concentration treatment, the GPx activity was less than the original of BCKC. The results implicated, on the GPx activity
of BCKCin vitro, the ranking of width range of the most suitable Se concentration for nutrition curve of the three Se formes is Nano-Se>Se-Met>Na2SeO3.
Foundation item: Supported by the Key Item of the Science and Technology Bureau of Zhejiang Province (021122680)
Biography: Xu Bao-hua (1966-), male, Associate professor, Ph. D. research direction: nanobiology and animal nutrition. |
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Keywords: | Nano-Se GPx activity broiler chick kidney cells (BCKC) cell culture |
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