大别山区葛根黄酮对四氧嘧啶糖尿病模型小鼠糖脂代谢的影响

为研究葛根黄酮(RPF)对糖尿病模型小鼠糖脂代谢的影响及其可能的作用机制,采用四氧嘧啶(ALX,220mg/kg)腹腔注射建立糖尿病小鼠模型。RPF连续灌胃15d后摘眼球取血,分离血清,测定血糖、血清甘油三酯(TG)、总胆固醇(TC)、一氧化氮(NO)和一氧化氮合酶(NOS)水平;取肝、肾脏制成10%匀浆测定其NO和NOS水平;观察胰腺病理学形态变化。结果显示RPF能降低ALX诱导的糖尿病模型小鼠血糖、血清和肝脏中NO和NOS。其降糖机制可能与降低血清、肝脏的NO和NOS水平有关。

Effects of Radix Pueraria Flavonoids in Da Bie Shan Zone on Blood Glucose and Lipid Level of Diabetic Mice Induced by Alloxan

To evaluate the effects of Radix Pueraria Flavonoids on blood glucose and lipid level of diabetic mice induced by alloxan and study the hypoglycemic activity of Radix Pueraria Flavonoids and the mechanism.Injecting alloxan（220 mg/kg） into its abdominal cavity to set animal model of diabetes.After 15d administration of RPF,blood glucose were determined and blood serum was drawn to determine TG,TC,NO and NOS.Liver and kidney were drawn to determine NO and NOS.Pancreatic pathology was studied with morphological method.Compared with diabetic model group,the results show that RPF could decrease the blood glucose level and lower NO,NOS in blood serum and liver.The mechanisms of hypoglycemic activity of RPF may be related to lowering NO and NOS in blood serum and liver.