MNV感染对乙肝疫苗效价评价的影响

目的 研究小鼠诺如病毒(murine norovirus,MNV)感染实验小鼠对乙肝疫苗效价评价的影响.方法 将BALB/c小鼠分为对照组和感染组,感染组经口灌胃MNV,对照组灌胃生理盐水,参照《中国药典》2015年第三部乙肝疫苗效价测定方法,将乙肝疫苗分为高、中、低3个剂量组,分别免疫对照组和感染组小鼠,于免疫后第7...

Influence of MNV Infection on the Evaluation of Hepatitis B Vaccine Titer

Abstract: Objective To study the effect of laboratory mice infected with MNV on the evaluation of the titer of hepatitis B vaccine. Method The BALB / c mice were divided into control and infection groups. The infected group was orally administered MNV and the control group was orally administered with saline. According to the Chinese Pharmacopoeia 2015 Part Ⅲ Hepatitis B vaccine titer determination method, hepatitis B vaccine was divided into high, medium and low dose groups and immunized the control group and the infected group mice respectively. Blood was collected on day 7, 14, 21, 28, and 35 after immunization to determine the hepatitis B surface antibody ( HbsAb ) . The ED50 values at each time point of immunization of the control group and the infection group were calculated and compared. Chi-square test was used to compare the difference of HbsAb positive rate between the control group and the infected group. Result The ED50 values of the control group immunized with hepatitis B vaccine on day 14, 21, 28, and 35 were 0. 55, 0. 55, 0. 28, and 0. 24 μg / mL, and those in the infected group were 0. 90, 0. 54, 0. 32, and 0. 32 μg / mL, respectively. On the day 21, the ED50values of the two groups were close, and the ED50 values of the control group at the remaining three time points were lower than those of the infected group. After chi-square test, there was no significant difference in the overall positive rate of HbsAb between the control group and the infected group at all time points ( P > 0. 05) . Further comparison revealed that on the day 28 and day 35 of immunization with hepatitis B vaccine, the number of animals with HbsAb immunized with high-dose hepatitis B vaccine in the control group was significantly higher than that in the infected group ( P < 0. 05 ) ; On the day 14 after immunization, the number of HbsAb-positive animals immunized with the middle-dose hepatitis B vaccine in the control group was significantly higher than that in the infection group ( P<0. 05) . Conclusion To determine the hepatitis B vaccine titer using MNV-positive mice will result in lower result.