2型糖尿病视网膜病变大鼠模型建立

目的 建立2型糖尿病视网膜病变大鼠模型.方法 80只雄性SD大鼠随机分成两组:空白对照组(普通饲料)及T2DM组(高脂高糖饲料喂养4周联合30 mg/kg链脲佐菌素腹腔注射).链脲佐菌素注射后观察大鼠一般情况、每周测量体质量、随机血糖、空腹血糖、空腹血清胰岛素等指标,计算并分析胰岛抵抗指数(HOMA-IR).分别在血糖...

Establishment of Type 2 Diabetic Retinopathy Rat Model

Abstract: Objective To establish a rat model of type 2 diabetic retinopathy. Method Eighty male SD rats were randomly divided into 2 groups. The blank control group ( n = 40) was fed ordinary diet and the type 2 diabetes group ( n = 40) was fed high-fat and high-sugar diet for 4 weeks with 30 mg / kg streptozotocin ( STZ) intraperitoneal injection. General condition, body weight, random blood glucose, fasting blood glucose and fasting serum insulin ( FINS) were measured weekly after STZ injection and HOMA-IR was calculated and analyzed. At the 3rd, 4th, 5th and 6th months after blood glucose stabilization, 10 rats from each group were anesthetized and pupil dilated and underwent fundus fluorescein angiography and pathological morphology examination to observe the retinal changes of type 2 diabetic rats. Result At the 1st week after STZ injection, rats in the type 2 diabetes( T2DM) group showed diabetes symptoms of polydipsia, polydipsia, polyuria and weight loss. With the progress of disease course, the general condition, body weight, random blood glucose and HOMA-IR of rats in the T2DM group and the blank control group were significantly changed, and the differences were statistically significant. Fundus fluorescein angiography was performed in the type 2 diabetic rats at 3 months: fundus was clear, retinal vessels were tortuous, microaneurysms and a small amount of spotty strong fluorescein were observed; pathological morphological changes: the cells in each layer were disordered, with dilated blood vessels in the ganglion cell layer, and the number of cells in the inner and outer nuclear layers was reduced and sparsely arranged. With the prolongation of the course of disease, fundus fluorescein angiography and pathological changes gradually became more serious at the 4, 5 and 6 months. Conclusion A rat model of type 2 diabetic retinopathy was established, which provides an ideal laboratory animal model for pathogenesis and pharmacodynamics.