广西巴马小型猪雄性初情期和精液性状的分析

目的探讨广西巴马小型猪雄性初情期和精液品质性状。方法将15、20、25、26、27、28、29、30、45、55、62、75日龄巴马小型猪睾丸制作成切片,检查其曲细精管中是否已经产生精子;采集巴马小型猪精液,检测其物理性状和化学成分。结果初步判断雄性巴马小型猪的初情期为45日龄左右。巴马小型猪精液的主要物理性状:射精量107.5 mL±39.0 mL、酸碱度7.6±0.3、精子密度0.732亿/mL、精子活率0.754±0.079、精子活力0.376±0.168、精子畸形率0.256±0.159。主要化学成分:Na+1.56 mg±0.13 mg/mL、K+0.78 mg±0.06 mg/mL、Cl-1.62 mg±0.18 mg/mL、磷脂8.7 mg±0.2 mg/100 mL、果糖117.4 mg±81.0 mg/100 mL、柠檬酸45.6 mg±77.6 mg/100 mL。

Analysis of Prepuberty and Semen Characteristics of Male Guangxi Bama Minipig

The objectives of this study were to examine the prepuberty and semen characteristics of male Guangxi Bama minipig.Testes of 15,20,25,26,27, 28,29,30,45,55,62,75 days old Bama minipig were formalin-fixed and paraffin-embedded and stained with hematoxylin and eosin,and were examined if sperms exist.Semen of Bama minipig were collected and sent to Guangxi Center for analysis and test research to analyze its physical characteristics and chemical compositions.The results showed that:(1) the prepubertal age of male Bama minipig is preliminarily determined to be 45 days old approximately;(2)the physical characteristics(ejaculate volume 107.5 mL±39.0 mL,pH 7.6±0.3,osmolarity 316±3 mOsm,sperm viability 0.754±0.079,motility 0.376±0.168,abnormality 0.256±0.159,concentration 73.2×10~6/mL) and chemical compositions(DNA 127.4 μg±8.0 μg/mL,protein 6.0 g±0.7 g/100 mL,phospholipids 8.7 mg±0.2 mg/100 mL,fructose 117.4 mg±81.0 mg/100 mL,prostaglandin 3.6 pg±0.8 pg/ mL,citric acid 45.6 mg±77.6 mg/100 mL,Na~+ 1.56 mg±0.13 mg/mL,K~+ 0.78 mg±0.06 mg/mL,Cl~-1.62 mg±0.18 mg/mL)of Bama minipig semen were analyzed.