27个菜心品种遗传多样性的ISSR分析

利用ISSR技术对27个菜心品种的遗传多样性进行了分析.结果表明,12条引物共检测到103个可重复位点,其中多态位点58个,多态位点百分率(P)为56.31%.平均多样性指数(1)为0.229,遗传相似系数(H)为0.144,观察等位基因数(Na)和有效等位基因数(Ne)分别为1.563和1.225,遗传距离(GD)的范围在0.029～0.344之间,表明菜心遗传多样性较低.经UPGMA聚类分析,将27个菜心品种分为六大类群.

ISSR Analysis of Genetic Diversity of 27 Flowering Chinese Cabbage

Genetic diversity of 27 flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tssen. et Lee) cultivars were analyzed with inter--simple sequence repeat (ISSR) molecular mark-ers. A total of 103 loci were detected using 12 ISSR primers, of which 58 were polymorphic, the percent-age of polymorphic loci (p) being 56.31%. Shannon's information index (I) and Nei's gene diversity (H) were 0.229 and 0.144, respectively, observed number of alleles (Na) and effective number of alleles (Ne) were 1.563 and 1.225, respectively, and the genetic distance ranged from 0.029 to 0.344, indicating that the genetic diversity of flowering Chinese cabbage was relatively low. UPGMA cluster analysis clustered the 27 cultivars into six groups.