Messenger RNA differential display strategies in birds and amphibians |
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Authors: | Simon H G |
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Institution: | (1) Department of Pediatrics, Northwestern University Medical School, CMIER-Developmental Systems Biology, 2300 Children's Plaza M/C 204, Chicago, Illinois 60614 (USA), Fax + 1 773 880 8266, e-mail: hgsimon@northwestern.edu, US |
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Abstract: | As an inroad to the discovery of genes involved in important biological activities, various techniques have been developed
for detecting genes based on their expression levels. Arbitrary amplification of different messenger RNA (mRNA) populations
and their comparison on display autoradiograms made mRNA differential display one of the most straightforward approaches to
identification of differentially regulated mRNAs. Over the past decade this strategy has been employed in many in vitro and
in vivo systems. The use of the method in bird and amphibian model systems is reviewed here, emphasizing several unique combinations
of model system and design of differential display screen. |
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Keywords: | , Differential gene expression, song system, circadian rhythm, limb regeneration, limb identity, birds, amphibia, |
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