Molecular cloning and nucleotide sequence of classical swine fever virus strain Shimen |
| |
Authors: | Huang Qianhua Zhang Chuyu Wang Jiafu Wang Ning Fu Liezhen |
| |
Institution: | (1) College of Life Science, Wuhan University, 430072 Wuhan, China |
| |
Abstract: | According to the previously published CSFV sequences, 18 paris of partially overlapping primers which span the entire genome
of CSFV strain Shimen were designed and synthesized. Each cDNA fragment of strain Shimen was amplified by RT-PCR method from
the anticoagulant blood of strain Shimen infected pig. The PCR fragments were cloned into pGEM-T vector respectively and sequenced.
The results show that we have obtained the nucleotide sequence of strain Shimen. The viral RNA consists of 12 297 nucleotides
including noncoding regions of 373 and 227 bases at the 5′ and 3′ end, respectively, and a single large open reading frame
spanning 11 697 nucleotides in the middle, which encodes an amino acid sequence of 3 989 residues with a calculated molecular
weight of 437.6×103. The precisely sequencing of 5′ and 3′ termini is undertaking.
Supported by the National Pandeng Project
Huang Qianhua: born in 1968. Graduate student |
| |
Keywords: | classical swine fever virus strain Shimen cloning sequencing |
本文献已被 SpringerLink 等数据库收录! |