采用气相色谱-质谱法分析药对麻黄-羌活的挥发油

采用气相色谱-质谱(GC-MS)法分离测定了药对麻黄-羌活、单味药麻黄和羌活的挥发油,并对其重叠色谱峰采用化学计量学解析法进行了分辨,得到药对和各单味药的纯色谱曲线和质谱。药对麻黄-羌活、单味药麻黄和羌活分辨出的色谱峰,通过质谱库对其进行定性,分别得到58,51和52个定性结果,占总含量的83.75%,88.70%和80.79%。实验结果表明:药对麻黄-羌活中挥发油化学成分有40种来自羌活,13种来自麻黄,新产生了10种化合物。

Determination of volatile components in Herba Ephedrae-Rhizoma seu Radix Notopeterygii by gas chromatography-mass spectrometry

Determination of volatile components in drug pair(DP) Herba Ephedrae-Rhizoma seu Radix Notopeterygii(HE-RsRN),single drug HE and RsRN was performed using two-dimensional gas chromatography-mass spectrometry(GC-MS) data coupled with chemometric resolution method(CRM).With the help of CRM,the two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound.Using these methods to process two-dimensional data,51,52,and 58 volatile chemical components in essential oil of HE,RsRN,and DP HE-RsRN were respectively determined qualitatively and quantitatively,accounting for 88.70%,80.79%,and 83.75% total contents of volatile oil of HE,RsRN,and DP HE-RsRN respectively.It is further demonstrated that 40 and 13 volatile components of DP HE-RsRN are from that of single drug RsRN and HE respectively,and 10 new volatile components in DP HE-RsRN are produced because of the chemical reactions and physical changes occurred in the process of decocting two single drugs.