Interphase differential sensitivity to protein synthesis inhibition

Summary The effect of protein synthesis inhibition during interphase ofAllium cepa L. root meristem cells was studied. Anisomycin and cycloheximide were used in intermittent treatments during interphase of a synchronous cell subpopulation labelled as binucleate by caffeine, and the delays in reaching prophase were recorded. High sensitivity to protein synthesis inhibition was detected in G₁ and in the S/G₂ boundary, while protein synthesis in most of the S and G₂ phase was not required for the normal timing of next prophase.