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东亚钳蝎蝎毒蛋白的免疫学研究
引用本文:张继东,房海,陈翠珍,李双安,李佩国,冯治新.东亚钳蝎蝎毒蛋白的免疫学研究[J].河北科技师范学院学报,1995(4).
作者姓名:张继东  房海  陈翠珍  李双安  李佩国  冯治新
作者单位:河北农业技术师范学院动物科学系
摘    要:应用电泳技术和免疫检测技术对我国分布最广、数量最多的东亚钳蝎的蝎毒蛋白进行了试验研究。结果表明:各蛋白组分能够有效地刺激家兔产生特异性抗体。应用琼脂扩散试验、免疫电泳试验、琼脂单相辐射免疫扩散试验、对流免疫电泳试验等试验方法对东亚钳蝎蝎毒进行检测是可行的。本研究可成为快速、灵敏、准确的东亚钳蝎蝎毒蛋白检测技术。

关 键 词:东亚钳蝎,蝎毒,电泳,免疫检测技术

IMMUNOASSAYS OF FRACTIONS OF VENOM FROM EAST-ASIA SCORPION(BUTHUS MARTENSII)
Zhang Jidong, Fang Hai ,Chen Cuizhen ,Li Shuangan ,Li Peiguo ,Feng Zhixin.IMMUNOASSAYS OF FRACTIONS OF VENOM FROM EAST-ASIA SCORPION(BUTHUS MARTENSII)[J].Journal of Hebei Normal University of Science & Technology,1995(4).
Authors:Zhang Jidong  Fang Hai  Chen Cuizhen  Li Shuangan  Li Peiguo  Feng Zhixin
Abstract:The crude venom from the East-Asia scorpion(Buthus martensii)being largest innumber and widespread in China,was investigated by electrophoreses and immunoas-says. The results showed that specific antibadies against the taxins from the East-Asiascorpion were effectively producted in rabbit. It was available to examin the differentcomponents of the venom from the East-Asia scorpion by means of agar double diffisiontest,immunoelectrophoresis,simple agar immuno-diffision test,counter immunoelec-trophoresis,and consequently all the trchniques to be used in the esperiments above wereproved to be rapid,sensitive and accurate.
Keywords:East-Asia scorpion  venom  electrophoresis  immunoassay
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