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| Chicken QTL mapping by multiplex PCR | |
| 作者姓名： | HUANG Yinhu HU Xiaoxiang DENGXuemei XUWeizhuo LI Ning FENG Jidong SUN Han WU Changxin |
| 作者单位： | 1.State Key Laboratory for Agribiotechnology, China Agricultural University' Beijing 100094' China;2.College of Animal Science and Technology' Jiangxi Agricultural University' Nanchang 330045' China,State Key Laboratory for Agribiotechnology, China Agricultural University' Beijing 100094' China,College of Animal Science and Technology'China Agricultural University. Beijing 100094. China,College of Animal Science and Technology'China Agricultural University. Beijing 100094. China,State Key Laboratory for Agribiotechnology, China Agricultural University' Beijing 100094' China,State Key Laboratory for Agribiotechnology, China Agricultural University' Beijing 100094' China,College of Animal Science and Technology' Jiangxi Agricultural University' Nanchang 330045' China,College of Animal Science and Technology'China Agricultural University. Beijing 100094. China |
| 基金项目： | 国家自然科学基金，国家重点基础研究发展计划(973计划) |
| 摘 要： | To facilitate rapid determination of the chromosomal location of quantitative trait loci' the current approaches to gene mapping are improved using a multiplex PCR technique. The high-throughput linkage analysis method described here allows selection of 178 from 328 microsatellite markers through the multiplex PCR method combined with the semi-automatic fluorescence-labeled DNA analysis technology. Those polymorphism markers are distributed on 23 autosomes and one sex chromosome (chromosome Z). covering 3080cM genetic distance. The average marker density is 18cM. dispersed into 30 different sets. These selected polymorphism microsatellite markers segregate with the family members, following the Mendel's heritage laws, and are very useful for chicken linkage map analysis as well as for the research on some important economic quantitative characters of chicken. |
| 关 键 词： | chicken genomic scanning multiplex PCR. microsatellite marker genotype. |
Chicken QTL mapping by multiplex PCR |
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| HUANG Yinhu,HU Xiaoxiang,DENGXuemei,XUWeizhuo,LI Ning,FENG Jidong,SUN Han,WU Changxin.Chicken QTL mapping by multiplex PCR[J].Progress in Natural Science,2002,12(1):73-76. | |
| Authors: | HUANG Yinhua Hu Xiaoxiang DENG Xuemei XU Weizhuo LI Ning FENG Jidong SUN Han WU Changxin |
| Institution: | 1. State Key Laboratory for Agribiotechnology, China Agricultural University, Beijing 100094, China; College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China 2. State Key Laboratory for Agribiotechnology, China Agricultural University, Beijing 100094, China 3. College of Animal Science and Technology,China Agricultural University, Beijing 100094, China 4. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China |
| Abstract: | To facilitate rapid determination of the chromosomal location of quantitative trait loci' the current approaches to gene mapping are improved using a multiplex PCR technique. The high-throughput linkage analysis method described here allows selection of 178 from 328 microsatellite markers through the multiplex PCR method combined with the semi-automatic fluorescence-labeled DNA analysis technology. Those polymorphism markers are distributed on 23 autosomes and one sex chromosome (chromosome Z). covering 3080cM genetic distance. The average marker density is 18cM. dispersed into 30 different sets. These selected polymorphism microsatellite markers segregate with the family members, following the Mendel's heritage laws, and are very useful for chicken linkage map analysis as well as for the research on some important economic quantitative characters of chicken. |
| Keywords: | chicken genomic scanning multiplex PCR microsatellite marker genotype |
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