| 高聚物反相填料在分离纯化莱菔硫烷中的应用 |
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| 引用本文： | 张胜智,闫旭,梁浩,袁其朋.高聚物反相填料在分离纯化莱菔硫烷中的应用[J].北京化工大学学报(自然科学版),2012,39(1):72-76. |
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| 作者姓名： | 张胜智 闫旭 梁浩 袁其朋 |
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| 作者单位： | 北京化工大学生命科学与技术学院,北京,100029;北京化工大学生命科学与技术学院,北京,100029;北京化工大学生命科学与技术学院,北京,100029;北京化工大学生命科学与技术学院,北京,100029 |
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| 基金项目： | 国家自然科学基金(20776009/20976009) |
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| 摘 要： | 采用高聚物反相填料装填的半制备柱(PST, 30μm, 300mm×10mm ID)对莱菔硫烷进行分离纯化，优化了色谱分离参数，确定最佳流动相为30%乙腈-水溶液，流速为2mL/min等度洗脱，检测波长254nm，进样量不超过20mg时，收率在61%以上，纯度可达90%。采用中心切割的方式收集，获得的产品纯度可达90%以上。
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| 关 键 词： | 高聚物 反相填料 色谱分离 莱菔硫烷 |
| 收稿时间： | 2011-05-05 |
The application of polystyrene-based resin reversed phase chromatography in the separation of sulforaphane |
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| ZHANG ShengZhi YAN Xu LIANG Hao YUAN QiPeng.The application of polystyrene-based resin reversed phase chromatography in the separation of sulforaphane[J].Journal of Beijing University of Chemical Technology,2012,39(1):72-76. |
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| Authors: | ZHANG ShengZhi YAN Xu LIANG Hao YUAN QiPeng |
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| Institution: | College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China |
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| Abstract: | Sulforaphane has been separated and purified by polystyrene-based reversed phase chromatography(PST,30 μm,300 mm×10 mm ID).The preparative separation conditions were optimized and the optimum parameters were found to be 30% acetonitrile in water as the mobile phase,and a flow-rate of 2 mL/min in the isocratic elution mode.The highest purity of sulforaphane was 90% with a loading of 20 mg.The yield was more than 61%.With the use of overloading conditions and the heart-cutting method,the purity of sulforaphane exceeded 90%. |
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| Keywords: | polystyrene reversed phase chromatography chromatographic separation sulforaphane |
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